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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
27/06/2019 |
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Actualizado : |
27/06/2019 |
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Tipo de producción científica : |
Artículos en Revistas Agropecuarias |
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Autor : |
GIAMBIASI, M.; ARRUABARRENA, A.; BRITOS, A.; CASTILLO, A.; BERTALMIO, A.; ROLON, R.; BERTONI, E.; GONCALVEZ, L.; LAXAGUE, J.; ADALID, E.; VIGNALE, B.; RIVAS, F. |
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Afiliación : |
MARIO ALEJANDRO GIAMBIASI RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANA ARRUABARRENA PASCOVICH, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANA LUCIA BRITOS SILVA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ALICIA MARIA CASTILLO SALLE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANA MARIA BERTALMIO CASARIEGO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RODOLFO ROQUE ROLON RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ESTEFANY BERTONI CORREA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANA LUCIA GONCALVEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JULIO ALCIDES LAXAGUE BARNECHE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ELBIO ADALID GUTIÉRREZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; BEATRIZ VIGNALE, Estación Experimental Facultad de Agronomía Salto, UdelaR; CARLOS FERNANDO RIVAS GRELA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Creando las bases genéticas de la competitividad citrícola: tecnologías aplicadas a la creación de variedades sin semillas. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Revista INIA Uruguay, 2019, no. 57, p. 13-17. |
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Serie : |
(Revista INIA; 57) |
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ISSN : |
1510-9011 |
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Idioma : |
Español |
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Contenido : |
Con el objetivo de contribuir a una mejora en la competitividad de la cadena citrícola de nuestro país, el Programa de Mejoramiento Genético de Citrus de INIA
profundiza en estrategias y tecnologías hacia un proceso continuo de liberación de variedades de cítricos sin semillas. A la vez que se mejora la calidad del producto,
se favorecen diferentes aspectos de los sistemas productivos y se colabora con la reducción de los costos de producción. |
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Thesagro : |
CITRUS. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/12930/1/Revista-INIA-57-Junio-2019-p.13-17.pdf
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Marc : |
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022 $a1510-9011
100 1 $aGIAMBIASI, M.
245 $aCreando las bases genéticas de la competitividad citrícola$btecnologías aplicadas a la creación de variedades sin semillas.
260 $c2019
490 $a(Revista INIA; 57)
520 $aCon el objetivo de contribuir a una mejora en la competitividad de la cadena citrícola de nuestro país, el Programa de Mejoramiento Genético de Citrus de INIA profundiza en estrategias y tecnologías hacia un proceso continuo de liberación de variedades de cítricos sin semillas. A la vez que se mejora la calidad del producto, se favorecen diferentes aspectos de los sistemas productivos y se colabora con la reducción de los costos de producción.
650 $aCITRUS
700 1 $aARRUABARRENA, A.
700 1 $aBRITOS, A.
700 1 $aCASTILLO, A.
700 1 $aBERTALMIO, A.
700 1 $aROLON, R.
700 1 $aBERTONI, E.
700 1 $aGONCALVEZ, L.
700 1 $aLAXAGUE, J.
700 1 $aADALID, E.
700 1 $aVIGNALE, B.
700 1 $aRIVAS, F.
773 $tRevista INIA Uruguay, 2019, no. 57, p. 13-17.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
26/01/2024 |
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Actualizado : |
26/01/2024 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
PICCOLI, R.J.; GRUCHOUSKEI, L.; BENINCA, A.L.V.; MAURENTE BERÓN, M.; CHENG, A.C.; DE ANDRADE, J.A.; FACCIN, M.; GRZEGOZEVSKI, A.P.; DA SILVA, G.C.R.; FRANÇA, J.C.; FERNANDES, N.L.M.; DE CARVALHO, A.L.; VIOTT, A.M. |
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Afiliación : |
RONALDO JOSÉ PICCOLI, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; LEONARDO GRUCHOUSKEI, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; ANDRÉ LUIS VRIESMAN BENINCA, Animal Parasitology Laboratory, Federal University of Paraná, PR, Palotina, Brazil; MARINA MAURENTE BERÓN, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ARTHUR COLOMBARI CHENG, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; JOICE APARECIDA DE ANDRADE, Animal Parasitology Laboratory, Federal University of Paraná, PR, Palotina, Brazil; MAYANE FACCIN, Veterinary Pathobiology Department, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, United States; ALINE PATRÍCIA GRZEGOZEVSKI, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; GEÓRGIA CAROLINA ROHDEN DA SILVA, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; JAQUELINE COELHO FRANÇA, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; NELSON LUIS MELLO FERNANDES, Immunological Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; ANDERSON LUIZ DE CARVALHO, Veterinary Hospital, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; ALINE DE MARCO VIOTT, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil. |
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Título : |
Detection of Chlamydia sp. by fluorescence in situ hybridization (FISH) in histologic sections of the liver from exotic and native avian species. |
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Complemento del título : |
Original full article. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Brazilian Journal of Veterinary Pathology, 2023, Volume 16, Issue 3, Pages 159-166. https://10.0.94.6/bjvp.1983-0246.v16i3p159-166 -- OPEN ACCESS. |
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ISSN : |
1983-0246 |
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DOI : |
10.0.94.6/bjvp.1983-0246.v16i3p159-166 |
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Idioma : |
Inglés |
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Notas : |
Article history: Submitted: 27 July 2023, Accepted: 15 September 2023. -- Document type: Article Bronze Open Access. -- Correspondence: Viott, A.M.; Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; email:alinedemarco@yahoo.com.br -- |
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Contenido : |
ABSTRACT.- Chlamydiosis is a zoonotic disease that affects several animal species. Therefore, reliable detection techniques are essential for efficient control of the disease. Fluorescence in situ hybridization (FISH) for Chlamydia sp. was applied to 137 formalin-fixed, paraffin-embedded liver sections from native and exotic birds. The samples were divided into two groups: retrospective (n=57) and prospective (n=80). The probe was designed based on an annealing sequence that targets the Major Outer Membrane Protein coding gene. Livers previously confirmed for Chlamydia psittaci by polymerase chain reaction (PCR) and immunohistochemistry (IHC) were used as positive controls. Also, 47 randomly selected samples from the prospective group were submitted to PCR for Chlamydia psittaci for confirmation. From all 137 samples, 67% (92/137) were positive for Chlamydia sp. through FISH, from which 39% (36/57) and 61% (56/80) were from the retrospective and prospective groups, respectively. From the samples of the prospective group submitted for PCR confirmation, 83% (39/47) of them had a positive correlation with FISH results. When considering the number of targeted microorganisms per 400x field, 42,39% (39/92) of the samples had up to five microorganisms, 14,13% (13/92) had from six to ten, and 43,47% (40/92) of the samples had 11 or more microorganisms per field. FISH is a specific and reliable method to identify Chlamydia sp. in histologic sections of the liver, providing an additional tool for detecting avian chlamydiosis. © 2023, Brazilian Association of Veterinary Pathology. All rights reserved. MenosABSTRACT.- Chlamydiosis is a zoonotic disease that affects several animal species. Therefore, reliable detection techniques are essential for efficient control of the disease. Fluorescence in situ hybridization (FISH) for Chlamydia sp. was applied to 137 formalin-fixed, paraffin-embedded liver sections from native and exotic birds. The samples were divided into two groups: retrospective (n=57) and prospective (n=80). The probe was designed based on an annealing sequence that targets the Major Outer Membrane Protein coding gene. Livers previously confirmed for Chlamydia psittaci by polymerase chain reaction (PCR) and immunohistochemistry (IHC) were used as positive controls. Also, 47 randomly selected samples from the prospective group were submitted to PCR for Chlamydia psittaci for confirmation. From all 137 samples, 67% (92/137) were positive for Chlamydia sp. through FISH, from which 39% (36/57) and 61% (56/80) were from the retrospective and prospective groups, respectively. From the samples of the prospective group submitted for PCR confirmation, 83% (39/47) of them had a positive correlation with FISH results. When considering the number of targeted microorganisms per 400x field, 42,39% (39/92) of the samples had up to five microorganisms, 14,13% (13/92) had from six to ten, and 43,47% (40/92) of the samples had 11 or more microorganisms per field. FISH is a specific and reliable method to identify Chlamydia sp. in histologic sections of the liver, providing an additio... Presentar Todo |
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Palabras claves : |
Diagnosis; Immunohistochemistry; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA; Psittacosis; Zoonosis. |
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Asunto categoría : |
L10 Genética y mejoramiento animal |
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URL : |
https://bjvp.org.br/wp-content/uploads/2023/10/BJVP_v16n3_159-166.pdf
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Marc : |
LEADER 03124naa a2200361 a 4500
001 1064440
005 2024-01-26
008 2023 bl uuuu u00u1 u #d
022 $a1983-0246
024 7 $a10.0.94.6/bjvp.1983-0246.v16i3p159-166$2DOI
100 1 $aPICCOLI, R.J.
245 $aDetection of Chlamydia sp. by fluorescence in situ hybridization (FISH) in histologic sections of the liver from exotic and native avian species.$h[electronic resource]
260 $c2023
500 $aArticle history: Submitted: 27 July 2023, Accepted: 15 September 2023. -- Document type: Article Bronze Open Access. -- Correspondence: Viott, A.M.; Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; email:alinedemarco@yahoo.com.br --
520 $aABSTRACT.- Chlamydiosis is a zoonotic disease that affects several animal species. Therefore, reliable detection techniques are essential for efficient control of the disease. Fluorescence in situ hybridization (FISH) for Chlamydia sp. was applied to 137 formalin-fixed, paraffin-embedded liver sections from native and exotic birds. The samples were divided into two groups: retrospective (n=57) and prospective (n=80). The probe was designed based on an annealing sequence that targets the Major Outer Membrane Protein coding gene. Livers previously confirmed for Chlamydia psittaci by polymerase chain reaction (PCR) and immunohistochemistry (IHC) were used as positive controls. Also, 47 randomly selected samples from the prospective group were submitted to PCR for Chlamydia psittaci for confirmation. From all 137 samples, 67% (92/137) were positive for Chlamydia sp. through FISH, from which 39% (36/57) and 61% (56/80) were from the retrospective and prospective groups, respectively. From the samples of the prospective group submitted for PCR confirmation, 83% (39/47) of them had a positive correlation with FISH results. When considering the number of targeted microorganisms per 400x field, 42,39% (39/92) of the samples had up to five microorganisms, 14,13% (13/92) had from six to ten, and 43,47% (40/92) of the samples had 11 or more microorganisms per field. FISH is a specific and reliable method to identify Chlamydia sp. in histologic sections of the liver, providing an additional tool for detecting avian chlamydiosis. © 2023, Brazilian Association of Veterinary Pathology. All rights reserved.
653 $aDiagnosis
653 $aImmunohistochemistry
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA
653 $aPsittacosis
653 $aZoonosis
700 1 $aGRUCHOUSKEI, L.
700 1 $aBENINCA, A.L.V.
700 1 $aMAURENTE BERÓN, M.
700 1 $aCHENG, A.C.
700 1 $aDE ANDRADE, J.A.
700 1 $aFACCIN, M.
700 1 $aGRZEGOZEVSKI, A.P.
700 1 $aDA SILVA, G.C.R.
700 1 $aFRANÇA, J.C.
700 1 $aFERNANDES, N.L.M.
700 1 $aDE CARVALHO, A.L.
700 1 $aVIOTT, A.M.
773 $tBrazilian Journal of Veterinary Pathology, 2023, Volume 16, Issue 3, Pages 159-166. https://10.0.94.6/bjvp.1983-0246.v16i3p159-166 -- OPEN ACCESS.
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